Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||33153||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepAc5.1/V5-His B
- Backbone size w/o insert (bp) 5400
Vector typeInsect Expression, Retroviral
Selectable markersHygromycin, Blasticidin
Growth in Bacteria
Growth Strain(s)XL1 Blue
SpeciesD. melanogaster (fly)
Entrez GeneClk (a.k.a. Dmel_CG7391, CG7391, CLK, CLOCK, Dmel\CG7391, Jerk, Jrk, PAS1, bHLHe10, clk, clock, dCLK, dCLK/JRK, dCLOCK, dClck, dClk, dClock, dclk, jrk)
- Promoter Actin 5C
/ Fusion Proteins
- V5 (C terminal on backbone)
- 6xHIS (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (destroyed during cloning)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer AC5 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAC-dCLK was a gift from Michael Rosbash (Addgene plasmid # 33153 ; http://n2t.net/addgene:33153 ; RRID:Addgene_33153)
For your References section:Wild-type circadian rhythmicity is dependent on closely spaced E boxes in the Drosophila timeless promoter. McDonald MJ, Rosbash M, Emery P. Mol Cell Biol. 2001 Feb;21(4):1207-17. 10.1128/MCB.21.4.1207-1217.2001 PubMed 11158307