pSG5-FLAG-CaMKKalpha RP deletion
(Plasmid #33328)

• Depositing Lab: Anthony Means
• Publication: Green et al Cell Signal. 2011 Dec;23(12):2005-12. Epub 2011 Jul 23.

Backbone

• Vector backbone: pSG5
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 4100
• Modifications to backbone: Added blunt end cloning site which was lost during cloning.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Calcium/Calmodulin dependent protein kinase kinase alpha
• Alt name: CaMKKalpha
• Alt name: CaMKK1
• Species: R. norvegicus (rat)
• Insert Size (bp): 1500
• Mutation: Deleted RP domain (amino acids 167-189 missing)
• GenBank ID: NP_113850
• Entrez Gene: Camkk1
• Promoter: SV40
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: None (destroyed during cloning)
• 3′ cloning site: None (destroyed during cloning)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry