Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||33334||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerAddgene plasmid #33337
- Backbone size w/o insert (bp) 6575
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
SpeciesM. musculus (mouse)
Insert Size (bp)1200
Entrez GeneAtoh1 (a.k.a. Hath1, MATH-1, Math1, bHLHa14)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pLXSN 5' (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please note: Addgene sequencing results determined that the BglII and XhoI sites are missing from the 3' end of the MCS and were likely destroyed during cloning.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMSCV-Atoh1wt-IRES-RFP was a gift from Martine Roussel & Charles Sherr (Addgene plasmid # 33334 ; http://n2t.net/addgene:33334 ; RRID:Addgene_33334)
For your References section:Atoh1 inhibits neuronal differentiation and collaborates with Gli1 to generate medulloblastoma-initiating cells. Ayrault O, Zhao H, Zindy F, Qu C, Sherr CJ, Roussel MF. Cancer Res. 2010 Jul 1;70(13):5618-27. Epub 2010 Jun 1. 10.1158/0008-5472.CAN-09-3740 PubMed 20516124