pLJD vector
(Plasmid
#34595)
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Purpose(Empty Backbone)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 34595 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepLJD
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Backbone manufacturerLT Chow/UAB
- Backbone size (bp) 6600
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Vector typeMammalian Expression, Retroviral
- Promoter LTR
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Growth instructionsuse Kan at 50ug/ml
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Copy numberUnknown
Cloning Information
- 5′ sequencing primer pBABE-3 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byconstruct provided by LT Chow, Univ. of Alabama at Birmingham
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
doi:10.1101/gad.9.19.2335
Genes Dev. 1995 9: 2335-2349
S Cheng, D C Schmidt-Grimminger, T Murant, et al.
Differentiation-dependent up-regulation of the human papillomavirus E7 gene reactivates cellular DNA replication in suprabasal differentiated keratinocytes.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLJD vector was a gift from Michael Ruppert (Addgene plasmid # 34595 ; http://n2t.net/addgene:34595 ; RRID:Addgene_34595) -
For your References section:
A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context. Lin CC, Liu LZ, Addison JB, Wonderlin WF, Ivanov AV, Ruppert JM. Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25. 10.1128/MCB.01189-10 PubMed 21518959
Map uploaded by the depositor.
