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G273D HA-dynamin 1 pUHD10-3
(Plasmid #34689)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 34689 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dynamin 1
  • Species
    H. sapiens (human)
  • Mutation
    G273D
  • Entrez Gene
    DNM1 (a.k.a. DEE31, DNM, EIEE31)
  • Tag / Fusion Protein
    • HA (variant) (N terminal on insert)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    G273D HA-dynamin 1 pUHD10-3 was a gift from Sandra Schmid (Addgene plasmid # 34689 ; http://n2t.net/addgene:34689 ; RRID:Addgene_34689)
  • For your References section:

    Clathrin-independent pinocytosis is induced in cells overexpressing a temperature-sensitive mutant of dynamin. Damke H, Baba T, van der Bliek AM, Schmid SL. J Cell Biol. 1995 Oct;131(1):69-80. 10.1083/jcb.131.1.69 PubMed 7559787