pCFJ326 - operon for germline expression
(Plasmid #34875)

• Depositing Lab: Erik Jorgensen
• Publication: Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865.

Backbone

• Vector backbone: pDONRP2-P3R
• Vector type: Worm targeting

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: tbb-2 gld-1 operon
• Species: C. elegans (nematode)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This plasmid is a derivative of the plasmid pCM1.157 made by Chris Merritt in Geraldine Seydoux's lab. For a very nice discussion of how 3' UTRs (single or in operons) regulate expression in the germline please see: Merritt C, Rasoloson D, Ko D, Seydoux G. Curr Biol. 2008 Oct 14;18(19):1476-82. 3' UTRs are the primary regulators of gene expression in the C. elegans germline.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry