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IFI16-(192-393)-HinA-E222A
(Plasmid #35056)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 35056 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET15b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5700

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    induce culture at OD600=0.8 with 1mM IPTG, 15oC overnight
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IFI16-(192-393)-HinA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    603
  • Mutation
    Contains HinA domain: aa 192-393; E222A mutation
  • Entrez Gene
    IFI16 (a.k.a. IFNGIP1, PYHIN2)
  • Promoter T7
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Growth conditions for protein production: BL21DE3-Codon-plus-RIL

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    IFI16-(192-393)-HinA-E222A was a gift from Cheryl Arrowsmith (Addgene plasmid # 35056 ; http://n2t.net/addgene:35056 ; RRID:Addgene_35056)
  • For your References section:

    Interferon-inducible protein 16: insight into the interaction with tumor suppressor p53. Liao JC, Lam R, Brazda V, Duan S, Ravichandran M, Ma J, Xiao T, Tempel W, Zuo X, Wang YX, Chirgadze NY, Arrowsmith CH. Structure. 2011 Mar 9;19(3):418-29. 10.1016/j.str.2010.12.015 PubMed 21397192