pcDNA Myc DBC1
(Plasmid #35096)

• Depositing Lab: Osamu Hiraike
• Publication: Hiraike et al Br J Cancer. 2010 Mar 16;102(6):1061-7. Epub 2010 Feb 16.

Backbone

• Vector backbone: pcDNA 3
• Backbone manufacturer: Invitrogen
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: deleted in breast cancer 1
• Alt name: DBC1
• Species: H. sapiens (human)
• Insert Size (bp): 2769
• Entrez Gene: CCAR2 (a.k.a. DBC-1, DBC1, KIAA1967, NET35, p30 DBC, p30DBC)
• Promoter: CMV
• Tag / Fusion Protein:
  • Myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry