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pBID-UASC-G
(Plasmid #35202)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 35202 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBID-UASC-G
  • Backbone manufacturer
    Ji-Wu Wang and Brian McCabe
  • Backbone size (bp) 11145
  • Vector type
    Drosophila Transgene Expression
  • Promoter Drosophila Synthetic Core Promoter (DSCP)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Generation of ϕC31transgenic Drosophila for GAL4 driven expression. Gateway cloning cassette is flanked by gypsy insulator sequences to allow uniform transgene expression between insertion sites. 10 UAS binding sites, Drosophila Synthetic Core promoter(DSCP). Transgene selection using white gene. loxP site facilitates elimination of transgene markers via Cre recombinasemediated excision with ZH-attP landing sites. Ampicillin resistant. Chloramphenicol resistant for amplification.

Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBID-UASC-G was a gift from Brian McCabe (Addgene plasmid # 35202 ; http://n2t.net/addgene:35202 ; RRID:Addgene_35202)
  • For your References section:

    A modular toolset for recombination transgenesis and neurogenetic analysis of Drosophila. Wang JW, Beck ES, McCabe BD. PLoS One. 2012;7(7):e42102. Epub 2012 Jul 25. 10.1371/journal.pone.0042102 PubMed 22848718