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MIGS5.2 (FF576)
(Plasmid #35254)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 35254 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGreenII
  • Backbone size (bp) 8002
  • Modifications to backbone
    1) Addition of an expression cassette with the miR173, however with no promoter, this should be added by multi-site gateway; 2) No promoter for expression of the MIGS construct. Instead, a promoter of choice can be cloned using multi-site gateway. See original article: de felippes et all, 2012, the Plant Journal.
  • Vector type
    Plant Expression
  • Selectable markers
    kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Spectinomycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    1) Before recombination Chloramphenicol might be used for bacteria selection (resistance lost after recombination). 2) E. coli DB3.1 strain (or some other ccdB survival strain) should be used for the amplification of the plasmid due to the presence of the lethal ccdB gene. After recombination, any common strain, such as DH5alpha is recommended. 3) Due to pGreen backbone characteristics, the helper plasmid pSOUP is required for replication of MIGS vector in Agrobacterium. PSOUP can be requested directly at http://www.pgreen.ac.uk/.
  • Copy number
    High Copy

Resource Information

  • Terms and Licenses

Depositor Comments

MIGS vectors are based on pGREEN series:

Hellens, R.P., Edwards, E.A., Leyland, N.R., Bean, S. and Mullineaux, P.M. (2000)
pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant
transformation. Plant Mol. Biol., 42, 819-832.

http://www.pgreen.ac.uk/

obtained from John Innes Centre, UK

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MIGS5.2 (FF576) was a gift from Detlef Weigel (Addgene plasmid # 35254)
  • For your References section:

    MIGS: MiRNA Induced Gene Silencing. de Felippes FF, Wang JW, Weigel D. Plant J. 2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x. 10.1111/j.1365-313X.2011.04896.x PubMed 22211571