pGEX 4T1 ERbeta
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||35563||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepGEX 4T1
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameEstrogen Receptor beta
SpeciesH. sapiens (human)
Insert Size (bp)1592
Entrez GeneESR2 (a.k.a. ER-BETA, ESR-BETA, ESRB, ESTRB, Erb, NR3A2, ODG8)
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer NA
- 3′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGEX 4T1 ERbeta was a gift from Harish Srinivas (Addgene plasmid # 35563 ; http://n2t.net/addgene:35563 ; RRID:Addgene_35563)
For your References section:Ligand-independent antiapoptotic function of estrogen receptor-beta in lung cancer cells. Zhang G, Yanamala N, Lathrop KL, Zhang L, Klein-Seetharaman J, Srinivas H. Mol Endocrinol. 2010 Sep;24(9):1737-47. Epub 2010 Jul 21. 10.1210/me.2010-0125 PubMed 20660297