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(Plasmid #35575)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35575 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone size w/o insert (bp) 8800
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    slmb (a.k.a. Dmel_CG3412, BcDNA:GM02031, CG3412, Dmel\CG3412, FBXW1, MENE (3R)-B, MENE(3R)-B, SLIMB, SLMB, Slimb, Slimb/Beta-TRCP1, Slimb/beta-TRCP, Slimb/beta-TrCP, Slimb/betaTrCP, Slmb, beta-TrCP, betaTrCP, crd, ica, l(3)00295, shv, slimb, wel)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer caactactgaaatctgcc
  • 3′ sequencing primer ctctgtaggtagtttgtcc
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUAST_NSlmb-vhhGFP4 was a gift from Markus Affolter (Addgene plasmid # 35575 ; ; RRID:Addgene_35575)
  • For your References section:

    Fluorescent fusion protein knockout mediated by anti-GFP nanobody. Caussinus E, Kanca O, Affolter M. Nat Struct Mol Biol. 2011 Dec 11;19(1):117-21. doi: 10.1038/nsmb.2180. 10.1038/nsmb.2180 PubMed 22157958