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FoxO1-ADA-GFP
(Plasmid #35640)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 35640 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Modifications to backbone
    Delete MCS sequence between Nhe I and Kpn I. These two restriction sites are also destroyed.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FoxO1-ADA-GFP
  • Alt name
    Foxo1
  • Alt name
    Fkhr
  • Alt name
    forkhead box O1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1700
  • Mutation
    T24A, S253D and S316A mutations
  • Entrez Gene
    Foxo1 (a.k.a. Afxh, FKHR, Fkhr1, Foxo1a)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Myc (N terminal on backbone)
    • Flag (N terminal on insert)
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kpn I (destroyed during cloning)
  • 3′ cloning site BamH I (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EGFP-N; SV40pA-R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

E15G and G282R mutations are present in this insert but are not known to affect function. There are also K219R and L619P polymorphisms. Plasmid should still function as expected of the ADA.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FoxO1-ADA-GFP was a gift from Domenico Accili (Addgene plasmid # 35640 ; http://n2t.net/addgene:35640 ; RRID:Addgene_35640)
  • For your References section:

    Uncoupling of acetylation from phosphorylation regulates FoxO1 function independent of its subcellular localization. Qiang L, Banks AS, Accili D. J Biol Chem. 2010 Aug 27;285(35):27396-401. Epub 2010 Jun 2. 10.1074/jbc.M110.140228 PubMed 20519497