FoxO1-ADA-GFP
(Plasmid #35640)

• Depositing Lab: Domenico Accili
• Depositing Organization: Columbia University
  Located in the United States of America
• Publication: Qiang et al J Biol Chem. 2010 Aug 27;285(35):27396-401. Epub 2010 Jun 2.
• Citations: 4 Articles

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Modifications to backbone: Delete MCS sequence between Nhe I and Kpn I. These two restriction sites are also destroyed.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FoxO1-ADA-GFP
• Alt name: Foxo1
• Alt name: Fkhr
• Alt name: forkhead box O1
• Species: M. musculus (mouse)
• Insert Size (bp): 1700
• Mutation: T24A, S253D and S316A mutations
• Entrez Gene: Foxo1 (a.k.a. Afxh, FKHR, Fkhr1, Foxo1a)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Myc (N terminal on backbone)
  • Flag (N terminal on insert)
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Kpn I (destroyed during cloning)
• 3′ cloning site: BamH I (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EGFP-N; SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

E15G and G282R mutations are present in this insert but are not known to affect function. There are also K219R and L619P polymorphisms. Plasmid should still function as expected of the ADA.

DNA (Catalog # 35640-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 35640-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.