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pCMV-mCherry-MHC-IIA
(Plasmid #35687)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 35687 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP
  • Backbone manufacturer
    Clontech
  • Modifications to backbone
    EGFP replaced with mCherry [cloned from pRSET-Bm (Shaner et al., 2004)] between AgeI and HindIII.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MHC IIA
  • Alt name
    myosin-IIA heavy chain
  • Alt name
    NMHC IIA
  • Species
    H. sapiens (human)
  • Entrez Gene
    MYH9 (a.k.a. BDPLT6, DFNA17, EPSTS, FTNS, MATINS, MHA, NMHC-II-A, NMMHC-IIA, NMMHCA)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer mCherry-F
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-mCherry-MHC-IIA was a gift from Venkaiah Betapudi (Addgene plasmid # 35687 ; http://n2t.net/addgene:35687 ; RRID:Addgene_35687)
  • For your References section:

    Myosin-IIA heavy-chain phosphorylation regulates the motility of MDA-MB-231 carcinoma cells. Dulyaninova NG, House RP, Betapudi V, Bresnick AR. Mol Biol Cell. 2007 Aug;18(8):3144-55. Epub 2007 Jun 13. 10.1091/mbc.e06-11-1056 PubMed 17567956