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pEYFP-Syx2
(Plasmid #35703)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35703 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 35703-D50 50 μg of DNA in Tris buffer $495
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DNA 35703-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pEYFP-C
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Syx2
  • Alt name
    synectin-binding RhoA ex-change factor
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3213
  • Mutation
    R205K
  • Entrez Gene
    Plekhg5 (a.k.a. Syx)
  • Promoter CMV
  • Tag / Fusion Protein
    • EYFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This construct contains a R205K mutation when compared to GenBank ID NP_001004156.1 This mutation reflects the sequence of the original mouse cDNA clone and was not introduced erroneously. The plasmid is exactly as described in the associated publication.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEYFP-Syx2 was a gift from Arie Horowitz (Addgene plasmid # 35703 ; http://n2t.net/addgene:35703 ; RRID:Addgene_35703)

  • For your References section:

    A PDZ-binding motif as a critical determinant of Rho guanine exchange factor function and cell phenotype. Liu M, Horowitz A. Mol Biol Cell. 2006 Apr;17(4):1880-7. Epub 2006 Feb 8. 10.1091/mbc.e06-01-0002 PubMed 16467373
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