Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||35946||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepcDNA3.1 myc/his A
- Backbone size w/o insert (bp) 5500
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
SpeciesH. sapiens (human)
Insert Size (bp)7380
Entrez GeneLRRK2 (a.k.a. AURA17, DARDARIN, PARK8, RIPK7, ROCO2)
- Promoter CMV
/ Fusion Proteins
- Myc (C terminal on backbone)
- His (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV fwd (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Site directed mutagenesis was performed on Addgene Plasmid 17609: LRRK2-WT.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:LRRK2-K1906M was a gift from Zhaoyang Feng (Addgene plasmid # 35946 ; http://n2t.net/addgene:35946 ; RRID:Addgene_35946)
For your References section:Dysregulated LRRK2 signaling in response to endoplasmic reticulum stress leads to dopaminergic neuron degeneration in C. elegans. Yuan Y, Cao P, Smith MA, Kramp K, Huang Y, Hisamoto N, Matsumoto K, Hatzoglou M, Jin H, Feng Z. PLoS One. 2011;6(8):e22354. Epub 2011 Aug 3. 10.1371/journal.pone.0022354 PubMed 21857923