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pT2HLacZpA1I.7 (#59)
(Plasmid #35965)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 35965 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript II SK (+)
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Tie2 promoter
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Tek (a.k.a. Cd202b, Hyk, STK1, Tie-2, Tie2)

Gene/Insert 2

  • Gene/Insert name
    SDK LacZ

Gene/Insert 3

  • Gene/Insert name
    SV40 pA

Gene/Insert 4

  • Gene/Insert name
    Tie2 full enhancer
  • Species
    M. musculus (mouse)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Positive control DNA where LacZ is expressed under the control of Tie2 promoter and enhander using the longer enhancer fragment as indicated in the map. Insert DNA fragments can be excised by SalI digest.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT2HLacZpA1I.7 (#59) was a gift from Thomas Sato (Addgene plasmid # 35965 ; http://n2t.net/addgene:35965 ; RRID:Addgene_35965)
  • For your References section:

    Uniform vascular-endothelial-cell-specific gene expression in both embryonic and adult transgenic mice. Schlaeger TM, Bartunkova S, Lawitts JA, Teichmann G, Risau W, Deutsch U, Sato TN. Proc Natl Acad Sci U S A. 1997 Apr 1;94(7):3058-63. 10.1073/pnas.94.7.3058 PubMed 9096345