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pcDNA4-Myc-eIF3f
(Plasmid #35975)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35975 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 35975-D50 50 μg of DNA in Tris buffer $495
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DNA 35975-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pcDNA4
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5075
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eukaryotic translation initiation factor 3, subunit F
  • Alt name
    eIF3f; EIF3S5; eIF3-p47
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1073
  • GenBank ID
    NM_003754
  • Entrez Gene
    EIF3F (a.k.a. EIF3S5, MRT67, eIF3-p47)
  • Tag / Fusion Protein
    • Myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BstBI (not destroyed)
  • 5′ sequencing primer CMV promoter
  • 3′ sequencing primer BGH rev
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please see the following article for additional information on the use of this plasmid:
Valente et al., Mol Cell. 2009 Oct 23;36(2):279-89
https://www.ncbi.nlm.nih.gov/pubmed/19854136

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA4-Myc-eIF3f was a gift from Susana Valente (Addgene plasmid # 35975 ; http://n2t.net/addgene:35975 ; RRID:Addgene_35975)

  • For your References section:

    Inhibition of HIV-1 replication by eIF3f. Valente ST, Gilmartin GM, Mott C, Falkard B, Goff SP. Proc Natl Acad Sci U S A. 2009 Mar 17;106(11):4071-8. doi: 10.1073/pnas.0900557106. Epub 2009 Feb 23. 10.1073/pnas.0900557106 PubMed 19237569
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