pcDNA4-Myc-eIF3f
(Plasmid #35975)

• Depositing Lab: Susana Valente
• Publication: Valente et al Proc Natl Acad Sci U S A. 2009 Mar 17;106(11):4071-8. doi: 10.1073/pnas.0900557106. Epub 2009 Feb 23.

Backbone

• Vector backbone: pcDNA4
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5075
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: eukaryotic translation initiation factor 3, subunit F
• Alt name: eIF3f; EIF3S5; eIF3-p47
• Species: H. sapiens (human)
• Insert Size (bp): 1073
• GenBank ID: NM_003754
• Entrez Gene: EIF3F (a.k.a. EIF3S5, MRT67, eIF3-p47)
• Tag / Fusion Protein:
  • Myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: BstBI (not destroyed)
• 5′ sequencing primer: CMV promoter
• 3′ sequencing primer: BGH rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please see the following article for additional information on the use of this plasmid:
Valente et al., Mol Cell. 2009 Oct 23;36(2):279-89
https://www.ncbi.nlm.nih.gov/pubmed/19854136