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pCY 3090-06
(Plasmid #36233)


Item Catalog # Description Quantity Price (USD)
Plasmid 36233 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pCY 3090-02
  • Vector type
    Yeast cassette for PCR and homologous recombination
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    DH5alpha used for DNA recovery of plasmid (i.e. DNA preps).
  • Copy number


  • Gene/Insert name
  • Insert Size (bp)
  • Tag / Fusion Protein
    • yEpolylinker-mCherry-URA3 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site SacI (unknown if destroyed)
  • 5′ sequencing primer CACCATCGTGGAACAGTACG
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    URA3 was amplified from pRS316 (Sikorski, R. S. and Hieter, P. (1989). A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122, 19-27.); however, parental vector contains mCherry for which a MTA is required (R. Tsien lab) - please see:

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The minor discrepancies between the Addgene and author's sequence do not affect the plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCY 3090-06 was a gift from Anne Robinson (Addgene plasmid # 36233 ; ; RRID:Addgene_36233)
  • For your References section:

    Cassette series designed for live-cell imaging of proteins and high-resolution techniques in yeast. Young CL, Raden DL, Caplan JL, Czymmek KJ, Robinson AS. Yeast. 2012 Mar;29(3-4):119-36. doi: 10.1002/yea.2895. Epub 2012 Apr 4. 10.1002/yea.2895 PubMed 22473760