|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||36233||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepCY 3090-02
Vector typeYeast cassette for PCR and homologous recombination
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth instructionsDH5alpha used for DNA recovery of plasmid (i.e. DNA preps).
Insert Size (bp)1220
/ Fusion Protein
- yEpolylinker-mCherry-URA3 (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (unknown if destroyed)
- 3′ cloning site SacI (unknown if destroyed)
- 5′ sequencing primer CACCATCGTGGAACAGTACG (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The minor discrepancies between the Addgene and author's sequence do not affect the plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCY 3090-06 was a gift from Anne Robinson (Addgene plasmid # 36233 ; http://n2t.net/addgene:36233 ; RRID:Addgene_36233)
For your References section:Cassette series designed for live-cell imaging of proteins and high-resolution techniques in yeast. Young CL, Raden DL, Caplan JL, Czymmek KJ, Robinson AS. Yeast. 2012 Mar;29(3-4):119-36. doi: 10.1002/yea.2895. Epub 2012 Apr 4. 10.1002/yea.2895 PubMed 22473760