|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||36328||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerVieira and Messing, 1991
- Backbone size (bp) 3868
Modifications to backboneA 778bp oriT (Mob) HaeII fragment from pSUP202 was cloned into pUK21 at an HaeII site upstream of the lac region.
Vector typeBacterial Expression ; Suicide vector for R. rubrum
- Promoter reduce strength lacZ
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer none
- 3′ sequencing primer LacI-R or LacZ-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Constructed by D. P. Lies and G. P. Roberts
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pUX19 was a gift from Gary Roberts (Addgene plasmid # 36328 ; http://n2t.net/addgene:36328 ; RRID:Addgene_36328)