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NL4-3 mCherry Luciferase
(Plasmid #44965)


Item Catalog # Description Quantity Price (USD)
Plasmid 44965 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    AIDS Research and Reference Reagent Program
  • Total vector size (bp) 12424
  • Vector type
  • Selectable markers
    mCherry, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    mCherry, Luciferase, IRES
  • Alt name
    HIV 1 reporter virus
  • Species
    Lentivirus, Human specific
  • Mutation
    S34C mutation in Nef
  • Tags / Fusion Proteins
    • mCherry with D212G (C terminal on insert)
    • Luciferase, IRES (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (destroyed during cloning)
  • 5′ sequencing primer from pNLENG1 5'
  • 3′ sequencing primer from pNLENG1 3'
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    pNL4-3 obtained from the AIDS Research and Reference Reagent Program pSicoRMS2 from Matt Spindler and Bruce Conklin, Gladstone Institute of Cardiovascular Disease
  • Articles Citing this Plasmid

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the D212G mutation in mCherry has no effect on the function of this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NL4-3 mCherry Luciferase was a gift from Warner Greene (Addgene plasmid # 44965 ; ; RRID:Addgene_44965)
  • For your References section:

    A flexible model of HIV-1 latency permitting evaluation of many primary CD4 T-cell reservoirs. Lassen KG, Hebbeler AM, Bhattacharyya D, Lobritz MA, Greene WC. PLoS One. 2012;7(1):e30176. doi: 10.1371/journal.pone.0030176. Epub 2012 Jan 24. 10.1371/journal.pone.0030176 PubMed 22291913