|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13036||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Copy numberHigh Copy
Gene/Insert namecat, sacB
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer Unknown (Common Sequencing Primers)
pKM154 is a derivative of pBR322 that had sequence between the EcoR1 site and NdeI site removed, with a NotI site inserted (pTP809).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pKM154 was a gift from Kenan Murphy (Addgene plasmid # 13036 ; http://n2t.net/addgene:13036 ; RRID:Addgene_13036)
For your References section:PCR-mediated gene replacement in Escherichia coli. Murphy KC, Campellone KG, Poteete AR. Gene. 2000 Apr 4. 246(1-2):321-30. 10.1016/S0378-1119(00)00071-8 PubMed 10767554