|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||32108||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
- Promoter imyc
- Cloning method Restriction Enzyme
- 5′ cloning site Pme (not destroyed)
- 3′ cloning site Hind III (not destroyed)
- 5′ sequencing primer pMS-seq1 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
This is an expression vector for an enhanced Flp recombinase to excise FRT-flanked inserts in mycobacteria.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMN234 was a gift from Michael Niederweis (Addgene plasmid # 32108 ; http://n2t.net/addgene:32108 ; RRID:Addgene_32108)
For your References section:Consecutive gene deletions in Mycobacterium smegmatis using the yeast FLP recombinase. Stephan J, Stemmer V, Niederweis M. Gene. 2004 Dec 8;343(1):181-90. 10.1016/j.gene.2004.08.028 PubMed 15563844