pML597
(Plasmid
#32235)
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 32235 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepMN234
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namemflpe
-
MutationM.tuberculosis codon optimized flpe gene
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Bam HI (not destroyed)
- 3′ cloning site Hind III (not destroyed)
- 5′ sequencing primer imyc_seq (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This contains a codon optimized flpe gene encoding an enhanced Flp recombinase for efficient excision of FRT-flanked inserts in mycobacteria.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pML597 was a gift from Michael Niederweis (Addgene plasmid # 32235 ; http://n2t.net/addgene:32235 ; RRID:Addgene_32235) -
For your References section:
Functional expression of the Flp recombinase in Mycobacterium bovis BCG. Song H, Niederweis M. Gene. 2007 Sep 15;399(2):112-9. Epub 2007 May 21. 10.1016/j.gene.2007.05.005 PubMed 17590537
