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TAL2423
(Plasmid #36824)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 36824 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    JDS71
  • Backbone manufacturer
    Joung lab (Addgene Plasmid # 32287)
  • Backbone size w/o insert (bp) 6447
  • Vector type
    Mammalian, T7 for in vitro mRNA synthesis

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SMAD2-TALEN_Right
  • Alt name
    SMAD2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1538
  • Entrez Gene
    Smad2 (a.k.a. 7120426M23Rik, Madh2, Madr2, Smad-2, mMad2)
  • Promoter CMV
  • Tags / Fusion Proteins
    • 3X Flag (N terminal on backbone)
    • WT FOKI (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (destroyed during cloning)
  • 3′ cloning site BsmBI (destroyed during cloning)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Target binding site: TCTTCACAACTGGCGGC

It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TAL2423 was a gift from Keith Joung (Addgene plasmid # 36824 ; http://n2t.net/addgene:36824 ; RRID:Addgene_36824)
  • For your References section:

    FLASH assembly of TALENs for high-throughput genome editing. Reyon D, Tsai SQ, Khayter C, Foden JA, Sander JD, Joung JK. Nat Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170. 10.1038/nbt.2170 PubMed 22484455