Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||37106||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7800
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
SpeciesH. sapiens (human)
Insert Size (bp)1600
Mutationaa 379-928 only; large pocket of human Rb with aa substitutions S780A, S788D, S795A, S807A, S811L, T821A, T826A
Entrez GeneRB1 (a.k.a. OSRC, PPP1R130, RB, p105-Rb, pRb, pp110)
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer LNCX
- 3′ sequencing primer IRES-R (Common Sequencing Primers)
Also known as PSM.7LP (Knudsen and Wang 1997 MCB 17:5771).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pQCXIH-PSM-Rb was a gift from Joseph Nevins (Addgene plasmid # 37106 ; http://n2t.net/addgene:37106 ; RRID:Addgene_37106)
For your References section:A role for Mediator complex subunit MED13L in Rb/E2F-induced growth arrest. Angus SP, Nevins JR. Oncogene. 2012 Jan 16. doi: 10.1038/onc.2011.622. 10.1038/onc.2011.622 PubMed 22249253