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pQCXIH-PSM-Rb
(Plasmid #37106)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 37106 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pQCXIH
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 7800
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rb
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1600
  • Mutation
    aa 379-928 only; large pocket of human Rb with aa substitutions S780A, S788D, S795A, S807A, S811L, T821A, T826A
  • Entrez Gene
    RB1 (a.k.a. OSRC, PPP1R130, RB, p105-Rb, pRb, pp110)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer LNCX
  • 3′ sequencing primer IRES-R
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Also known as PSM.7LP (Knudsen and Wang 1997 MCB 17:5771).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQCXIH-PSM-Rb was a gift from Joseph Nevins (Addgene plasmid # 37106 ; http://n2t.net/addgene:37106 ; RRID:Addgene_37106)
  • For your References section:

    A role for Mediator complex subunit MED13L in Rb/E2F-induced growth arrest. Angus SP, Nevins JR. Oncogene. 2012 Jan 16. doi: 10.1038/onc.2011.622. 10.1038/onc.2011.622 PubMed 22249253