|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||37131||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Insert Size (bp)710
MutationA44V/F84L/W145M/I165D/M167L/G202D compared to mOrange (but numbering relative to EGFP)
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site BglII (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLSSmOrange-C1 was a gift from Vladislav Verkhusha (Addgene plasmid # 37131 ; http://n2t.net/addgene:37131 ; RRID:Addgene_37131)
For your References section:An Orange Fluorescent Protein with a Large Stokes Shift for Single-Excitation Multicolor FCCS and FRET Imaging. Shcherbakova DM, Hink MA, Joosen L, Gadella TW, Verkhusha VV. J Am Chem Soc. 2012 Apr 24. 10.1021/ja3018972 PubMed 22486524