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UQ5827
(Bacterial strain #37174)

No maps are available for this item.

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Bacterial Strain 37174 Bacteria in agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUX3056
  • Modifications to backbone
    ~5-kb BamHI + XhoI fragment from pUX3009 cloned into BamHI + SalI-cut pEXT20.

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ~5-kb BamHI + XhoI fragment from pUX3009 cloned into BamHI + SalI-cut pEXT20

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This strain contains Roberts lab plasmid pUX3056 which contains ~5-kb BamHI + XhoI fragment from pUX3009 cloned into BamHI + SalI-cut pEXT20. Useful for mutagenesis and excision of the “d + e + f”
binding region.

For strain usage details see attached pdf. Please note that Addgene has not tested this plasmid functionally.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    UQ5827 was a gift from Gary Roberts (Addgene plasmid # 37174)