Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Bacterial Strain||37175||Bacteria in agar stab||1||$75|
This material is available to academics and nonprofits only.
Modifications to backbone~5-kb BamHI + XhoI fragment from pUX3010 cloned into BamHI + SalI-cut pEXT20.
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert name~5-kb BamHI + XhoI fragment from pUX3010 cloned into BamHI + SalI-cut pEXT20
- Cloning method Unknown
- 5′ sequencing primer NA
- 3′ sequencing primer pRcoM1bs-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
This strain contains Roberts lab plasmid pUX3057 which contains ~5-kb BamHI + XhoI fragment from pUX3010 cloned into BamHI + SalI-cut pEXT20. Useful for mutagenesis and excision of the “a-f”
For strain usage details see attached pdf. Please note that Addgene has not tested this strain functionally.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:UQ5828 was a gift from Gary Roberts (Addgene plasmid # 37175)