|Item||Catalog #||Description||Quantity||Price (USD)|
|Bacterial Strain||37177||Bacteria in agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepUX3242 and pUX2410
Modifications to backbonepUX3242 = pUX2996 reporter system with higher-affinity RcoM1 binding site at the “e” position (“e↑” binding site = 5′-TCCTACAGTTCACGCACGT-3′)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
Growth Strain(s)E. Coli UQ5853
Growth instructionsCopy number: pUX2410 High / pUX3242 Low
Gene/Insert namecoxM1′::lacZ reporter system with higher-affinity RcoM1 binding site at the "e" position AND RcoM1 with C-term 6xHIS
- Cloning method Unknown
- 5′ sequencing primer pEXT20-F1
- 3′ sequencing primer pRcoM1bs-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
This is Roberts lab strain UQ5853 (Addgene #37159) containing Roberts lab plasmids pUX3242 (pUX2996 with a coxM1′::lacZ reporter system such that ...vector DNA – BamHI (unique site) – higher-affinity binding site at the “e” position (“e↑” binding site = 5′-TCCTACAGTTCACGCACGT-3′) – coxM1′(with unique SalI site)::lacZ-> tfd <-KanR (with 5′-end unique XhoI site) – vector DNA <-SpR...) AND pUX2410 (for expression of Burkholderia xenovorans RcoM1 protein + C-terminal 6×His tag).
The strain is CmR, pUX3242 is KanR and SpecR and pUX2410 is AmpR. Can be grown in Amp + Kan.
For strain usage see attached pdf.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:UQ6139 was a gift from Gary Roberts (Addgene plasmid # 37177)