|Item||Catalog #||Description||Quantity||Price (USD)|
|Bacterial Strain||37180||Bacteria in agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepUX3297 AND pUX2410
Modifications to backbonepUX3297 = pUX3009 reporter system with an “e” binding site “TTnnnG” motif changed to “GGnnnG”
Growth in Bacteria
Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
Growth Strain(s)E. Coli UQ5853
Growth instructionsCopy number: pUX2410 High / pUX3297 Low
Gene/Insert namecoxM1′::lacZ reporter system of pUX3009 with “e” binding site “TTnnnG” motif changed to “GGnnnG” AND RcoM1 with C-term 6xHIS tag
- Cloning method Unknown
- 5′ sequencing primer pEXT20-F1
- 3′ sequencing primer pRcoM1bs-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
This is Roberts lab strain UQ5853 (Addgene #37159) containing Roberts lab plasmids pUX3297 (pUX3009 based coxM1′::lacZ reporter system with binding sites “d + e + f” with the “e” binding site “TTnnnG” motif changed to “GGnnnG;”) AND pUX2410 (for expression of Burkholderia xenovorans RcoM1 protein + C-terminal 6×His tag).
Strain is CmR, pUX3297 is KanR and SpecR, pUX2410 is AmpR. Can be grown in Amp + Kan.
For strain usage see attached pdf.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:UQ6211 was a gift from Gary Roberts (Addgene plasmid # 37180)