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ZO-2 shRNA 1609
(Plasmid #37209)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 37209 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSUPER
  • Backbone size w/o insert (bp) 3176
  • Total vector size (bp) 3200
  • Vector type
    Mammalian Expression, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dog ZO-2 shRNA
  • Alt name
    ZO-2
  • gRNA/shRNA sequence
    GCAGCAGTATTCCGACTAT
  • Species
    canine
  • Entrez Gene
    TJP2 (a.k.a. ZO-2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ZO-2 shRNA 1609 was a gift from James Anderson & Alan Fanning (Addgene plasmid # 37209 ; http://n2t.net/addgene:37209 ; RRID:Addgene_37209)
  • For your References section:

    ZO-1 stabilizes the tight junction solute barrier through coupling to the perijunctional cytoskeleton. Van Itallie CM, Fanning AS, Bridges A, Anderson JM. Mol Biol Cell. 2009 Sep;20(17):3930-40. Epub 2009 Jul 15. 10.1091/mbc.e09-04-0320 PubMed 19605556