pMf3tvtaulacZ
(Plasmid #37264)

• Depositing Lab: Patricia Labosky
• Publication: Kloetzli et al Genesis. 2001 Feb;29(2):60-71.

Backbone

• Vector backbone: pPNT
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 17000
• Modifications to backbone: The targeting vector was constructed using 129Sv/J DNA. A 3.5kb EcoR1 fragment was used as the 39 homology arm and subcloned into a modified version of pPNT with a Not1 site introduced into the Sal1 site for linearization. An 8.5 kb BamH1/BglII fragment was used as the 59 homology arm. A tau-lacZ-PGKneo cassette (generous gift of Peter Mombaerts) was inserted between these two arms to give the vector described in Kloetzli et al. This deletion construct results in the replacement of the entire coding region of Foxb1 with the tau-lacZ fusion gene and the PGKneo cassette. The resulting mutation is a null.
• Selectable markers: Neomycin (select with G418) ; thymidine kinase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Foxb1
• Alt name: Mf3
• Alt name: Fkh5
• Species: M. musculus (mouse)
• Insert Size (bp): 17000
• GenBank ID: 1927549
• Entrez Gene: Foxb1 (a.k.a. C43, Fkh5, Foxb1a, Foxb1b, Hfh-e5.1, Mf3, Twh)
• Tag / Fusion Protein:
  • taulacZ

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry