pCaShs-Tum
(Plasmid #37303)

• Depositing Lab: Norbert Perrimon
• Publication: Harrison et al EMBO J. 1995 Jun 15;14(12):2857-65.

Backbone

• Vector backbone: pCaShs-hopscotch
• Backbone manufacturer: Addgene plasmid# 37299
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: hopTum-1
• Alt name: Tumorous-lethal
• Alt name: Tum-1
• Species: D. melanogaster (fly)
• Mutation: Tul-l mutation is a G to A transversion at nucleotide 1641 resulting in the substitution of a Glu for a Gly at amino acid 341
• GenBank ID: AAA62441.1
• Entrez Gene: hop (a.k.a. Dmel_CG1594, 4, CG1594, Dm JAK, DmHD-160, Dmel\CG1594, HD-160, HOP, Hop, Hop1, Hop2, Hpo, JAK, Jak, L4, Tum, Tum-1, d-jak, hop/JAK, jak, l(1)10Be, l(1)G18, l(1)L4, l(1)hop, msvl)
• Promoter: Hsp70

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (unknown if destroyed)
• 3′ cloning site: XbaI (unknown if destroyed)
• 5′ sequencing primer: pCasper-hs (5'-GCAACTACTGAAATCTGCCAAG-3')

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Perrimon Lab plasmid #101

The pCaShs-Tum construct was generated by the replacement of a 220 bp SacII-BstEII fragment from pCaShs-hop (Addgene plasmid# 37299) with the same fragment of PCR-generated DNA from hopTum-1 hemizygous flies.

This mutation is a G to A transversion at nucleotide 1641 resulting in the substitution of a Glu for a Gly at amino acid 341.