pEEF1D-FLAG (pcDNA 3.1+) LG6-1
(Plasmid #37365)

• Depositing Lab: David Litchfield
• Publication: Gyenis et al J Proteome Res. 2011 Nov 4;10(11):4887-901. Epub 2011 Oct 13.

Backbone

• Vector backbone: pcDNA3.1+
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 6300
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EEF1D
• Alt name: elongation factor 1-delta
• Alt name: EEF1D transcript variant 2
• Species: H. sapiens (human)
• Insert Size (bp): 875
• GenBank ID: gi|25453472
• Entrez Gene: EEF1D (a.k.a. EF-1D, EF1D, FP1047)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry