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pT7-EGFP-C1-HsNot3
(Plasmid #37372)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 37372 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4780
  • Total vector size (bp) 7039
  • Modifications to backbone
    T7-promoter upstream of the EGFP-gene
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HsNot3
  • Alt name
    CNOT3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2259
  • GenBank ID
    NM_014516
  • Entrez Gene
    CNOT3 (a.k.a. LENG2, NOT3, NOT3H)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT7-EGFP-C1-HsNot3 was a gift from Elisa Izaurralde (Addgene plasmid # 37372 ; http://n2t.net/addgene:37372 ; RRID:Addgene_37372)
  • For your References section:

    GW182 proteins directly recruit cytoplasmic deadenylase complexes to miRNA targets. Braun JE, Huntzinger E, Fauser M, Izaurralde E. Mol Cell. 2011 Oct 7;44(1):120-33. 10.1016/j.molcel.2011.09.007 PubMed 21981923