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pAAV-EF1a-DIO-HB
(Plasmid #37452)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 37452 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

This item is currently unavailable outside the US without additional regulatory approval.
A non-refundable shipping export licensing fee of $85 is required to cover Addgene’s additional processing costs.

Backbone

  • Vector backbone
    AAV
  • Total vector size (bp) 5300
  • Vector type
    Adeno-associated vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Histone, 2A element, rabies B19 glycoprotein
  • Alt name
    histone
  • Alt name
    2A element
  • Alt name
    rabies B19 glycoprotein
  • Insert Size (bp)
    2700
  • Promoter EF1a
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsrG1/EcoRV (destroyed during cloning)
  • 3′ cloning site NheI/XbaI (destroyed during cloning)
  • 5′ sequencing primer EF1a_fwd
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that there are 2 mutations in Histone, D26G and V119I. These mutations should not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-EF1a-DIO-HB was a gift from Edward Callaway (Addgene plasmid # 37452 ; http://n2t.net/addgene:37452 ; RRID:Addgene_37452)