pKT0128-RAS2 C-term
(Plasmid
#37557)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 37557 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepKT0128
- Backbone size w/o insert (bp) 4749
- Total vector size (bp) 4787
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Vector typeBacterial Expression
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRas2 C-term (last 9 aa)
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)42
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsrGI (not destroyed)
- 3′ cloning site BsrGI (not destroyed)
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKT0128-RAS2 C-term was a gift from Wei-Lih Lee (Addgene plasmid # 37557 ; http://n2t.net/addgene:37557 ; RRID:Addgene_37557) -
For your References section:
A CAAX motif can compensate for the PH domain of Num1 for cortical dynein attachment. Tang X, Punch JJ, Lee WL. Cell Cycle. 2009 Oct 1;8(19):3182-90. Epub 2009 Oct 4. 10.4161/cc.8.19.9731 PubMed 19755860