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pNG2462
(Plasmid #37836)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 37836 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pPD49.26
  • Backbone manufacturer
    Fire Lab
  • Backbone size w/o insert (bp) 3410
  • Total vector size (bp) 9497
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Pnhx-2F13D12.6::YFP
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    3224
  • Entrez Gene
    F13D12.6 (a.k.a. CELE_F13D12.6)
  • Promoter nhx-2
  • Tag / Fusion Protein
    • YFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer CGCTCTCTCGTCTCTCTCTC
  • 3′ sequencing primer CGTGTCTTGTAGTTCCCGTCATC
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNG2462 was a gift from Gary Silverman (Addgene plasmid # 37836 ; http://n2t.net/addgene:37836 ; RRID:Addgene_37836)
  • For your References section:

    A pro-cathepsin L mutant is a luminal substrate for endoplasmic-reticulum-associated degradation in C. elegans. Miedel MT, Graf NJ, Stephen KE, Long OS, Pak SC, Perlmutter DH, Silverman GA, Luke CJ. PLoS One. 2012;7(7):e40145. Epub 2012 Jul 2. 10.1371/journal.pone.0040145 PubMed 22768338