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LYN11-FRB-CFP
(Plasmid #38003)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 38003 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 38003-D50 50 μg of DNA in Tris buffer $495
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DNA 38003-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pmCherry-N1
  • Backbone manufacturer
    CLONTECH
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5052
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    LYN
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    33
  • GenBank ID
    NM_002350.3
  • Entrez Gene
    LYN (a.k.a. JTK8, SAIDV, p53Lyn, p56Lyn)

Gene/Insert 2

  • Gene/Insert name
    MTOR
  • Insert Size (bp)
    276
  • GenBank ID
    NM_004958.3
  • Entrez Gene
    MTOR (a.k.a. FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS)
  • Tag / Fusion Protein
    • ECFP (C terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Tamas Balla

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LYN11-FRB-CFP was a gift from Robin Irvine (Addgene plasmid # 38003 ; http://n2t.net/addgene:38003 ; RRID:Addgene_38003)

  • For your References section:

    PI4P and PI(4,5)P2 Are Essential But Independent Lipid Determinants of Membrane Identity. Hammond GR, Fischer MJ, Anderson KE, Holdich J, Koteci A, Balla T, Irvine RF. Science. 2012 Jun 21. 10.1126/science.1222483 PubMed 22722250
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