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Spa-1 in pCCL-c-MNDU3c-(X2)-pgk-EGFP
(Plasmid #38154)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 38154 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number

Gene/Insert 1

  • Gene/Insert name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    SIPA1 (a.k.a. SPA1)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer MSCV_rev
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
  • Promoter PGK

Cloning Information for Gene/Insert 2

Resource Information

Depositor Comments

Construct made by Sun Yu Ji. Human Spa-1 was subcloned as a 3.3 kb NotI x SspI fragment into pSK digested with NotI x SmaI; it was isolated from this plasmid by EcoRI digestion. The 3.3 kb EcoRI fragment was ligated into pCCL-c-MNDU3-(X2)-PGK-EGFP digested with EcoRI. The orientation of the insert with respect to the MNDU3 promoter was checked by digestion with Kpn I. The construct makes untagged human SpaI from the MNDU3 promoter and EGFP from the PGK promoter.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Spa-1 in pCCL-c-MNDU3c-(X2)-pgk-EGFP was a gift from Nora Heisterkamp (Addgene plasmid # 38154 ; ; RRID:Addgene_38154)
  • For your References section:

    Bcr/Abl P190 interaction with Spa-1, a GTPase activating protein for the small GTPase Rap1. Yi SJ, Lee HT, Groffen J, Heisterkamp N. Int J Mol Med. 2008 Oct . 22(4):453-8. PubMed 18813851