Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.
We have implemented updates to our Transparency and Privacy Policy.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pSB4A5 JQ929582
(Plasmid #38169)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 38169 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 4485
  • Vector type
    Synthetic Biology ; BioBrick cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
  • Species
  • Insert Size (bp)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Superfolder GFP
  • Species
  • Insert Size (bp)

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This construct has attL and attR sites (LR).

The DNA data register in BP and LR states consist of a constitutive promoter (BBa_J23119) flanked by BP or LR recombination sites positioned in opposite orientation, resulting in DNA inversion when recombined (Figs 1 & 2 and Figs S1E and
S1F). A Rrnp T1 terminator (BBa_J61048) was added in reverse orientation upstream of
the promoter to prevent transcriptional read-through in the opposite orientation, so that in each state, only one fluorescent protein is visibly expressed. On each side of the recombination target, we cloned superfolder GFP and mKate2 under translational
control of measured strong RBSs (BIOFAB pilot C-dog project

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSB4A5 JQ929582 was a gift from Drew Endy (Addgene plasmid # 38169 ; ; RRID:Addgene_38169)
  • For your References section:

    Rewritable digital data storage in live cells via engineered control of recombination directionality. Bonnet J, Subsoontorn P, Endy D. Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 21. 10.1073/pnas.1202344109 PubMed 22615351