Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||38168||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4485
Vector typeSynthetic Biology ; BioBrick cloning vector
Growth in Bacteria
Copy numberLow Copy
Insert Size (bp)850
Gene/Insert nameSuperfolder GFP
Insert Size (bp)812
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer ccdB-Fwd
- 3′ sequencing primer EGFP-C (Common Sequencing Primers)
This construct has attB and attP sites (BP).
The DNA data register in BP and LR states consist of a constitutive promoter (BBa_J23119) flanked by BP or LR recombination sites positioned in opposite orientation, resulting in DNA inversion when recombined (Figs 1 & 2 and Figs S1E and
S1F). A Rrnp T1 terminator (BBa_J61048) was added in reverse orientation upstream of
the promoter to prevent transcriptional read-through in the opposite orientation, so that in each state, only one fluorescent protein is visibly expressed. On each side of the recombination target, we cloned superfolder GFP and mKate2 under translational
control of measured strong RBSs (BIOFAB pilot C-dog project http://biofab.org/data).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSB4A5 JQ929581 was a gift from Drew Endy (Addgene plasmid # 38168 ; http://n2t.net/addgene:38168 ; RRID:Addgene_38168)
For your References section:Rewritable digital data storage in live cells via engineered control of recombination directionality. Bonnet J, Subsoontorn P, Endy D. Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 21. 10.1073/pnas.1202344109 PubMed 22615351
Map uploaded by the depositor.