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pTH727-CEN-RLuc/staCFLuc
(Plasmid #38211)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 38211 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCENBEVY-U
  • Backbone size w/o insert (bp) 6616
  • Total vector size (bp) 9184
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Firefly Luciferase
  • Alt name
    FLuc
  • Species
    Photinus pyralis
  • Insert Size (bp)
    1644
  • Mutation
    The last three codons of the full-length Firefly luciferase gene have been deleted to yield a luciferase variant which is fully functional, but no longer localises to peroxisomes.
  • GenBank ID
    AF043450
  • Promoter TDH3 (=GPD)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer M13f
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Renilla luciferase
  • Alt name
    RLuc
  • Species
    Synthetic; Renilla reniformis
  • Insert Size (bp)
    942
  • GenBank ID
    AF043450.1
  • Promoter ADH1

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site XmaI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer M13r
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Both the FLuc and RLuc genes originated from a plasmid kindly provided by Dr David Bedwell (University of Alabama, Birmingham). Salas-Marco J, Bedwell DM (2004) Molecular and Cellular Biology 24:7769-7778.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTH727-CEN-RLuc/staCFLuc was a gift from Tobias von der Haar (Addgene plasmid # 38211 ; http://n2t.net/addgene:38211 ; RRID:Addgene_38211)
  • For your References section:

    Translation elongation can control translation initiation on eukaryotic mRNAs. Chu D, Kazana E, Bellanger N, Singh T, Tuite MF, von der Haar T. EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.1002/embj.201385651. Epub 2013 Dec 19. 10.1002/embj.201385651 PubMed 24357599