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(Plasmid #38775)


Item Catalog # Description Quantity Price (USD)
Plasmid 38775 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Actin promoter and intron
  • Alt name
  • Species
    Oryza sativa
  • Insert Size (bp)
  • Tags / Fusion Proteins
    • sGFP (S65T) (C terminal on backbone)
    • nos 3' terminator (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NcoI (not destroyed)
  • 5′ sequencing primer EGFP-N
  • 3′ sequencing primer EGFP-C
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There are several sequence discrepancies between Addgene's quality control sequence and the available NCBI reference sequence for actin promoter. These differences should not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAct1IsGFP-1 was a gift from Peggy Lemaux (Addgene plasmid # 38775 ; ; RRID:Addgene_38775)
  • For your References section:

    Production of transgenic tall fescue and red fescue plants by particle bombardment of mature seed-derived highly regenerative tissues. Cho MJ, Ha CD, Lemaux PG. Plant Cell Rep. 2000 Nov;19(11):1084-1089. doi: 10.1007/s002990000238. 10.1007/s002990000238 PubMed 30754774