pAct1IsGFP-1
(Plasmid #38775)

• Depositing Lab: Peggy Lemaux
• Publication: Cho et al Plant Cell Rep. 2000 Nov;19(11):1084-1089. doi: 10.1007/s002990000238.

Backbone

• Vector backbone: pBlueSGFP
• Total vector size (bp): 5335
• Vector type: Plant transformation

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Actin promoter and intron
• Alt name: Act1
• Species: Oryza sativa
• Insert Size (bp): 1350
• Tags / Fusion Proteins:
  • sGFP (S65T) (C terminal on backbone)
  • nos 3' terminator (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: NcoI (not destroyed)
• 5′ sequencing primer: EGFP-N
• 3′ sequencing primer: EGFP-C

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There are several sequence discrepancies between Addgene's quality control sequence and the available NCBI reference sequence for actin promoter. These differences should not affect function.