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pNestin-EGFP
(Plasmid #38777)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 38777 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 38777-D50 50 μg of DNA in Tris buffer $495
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DNA 38777-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pEGFP-1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    nestin 2nd intron enhancer with TK mini promoter
  • Species
    H. sapiens (human)
  • GenBank ID
    NG_012300.1
  • Entrez Gene
    NES (a.k.a. Nbla00170)
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNestin-EGFP was a gift from Wei Cui (Addgene plasmid # 38777 ; http://n2t.net/addgene:38777 ; RRID:Addgene_38777)

  • For your References section:

    Generation of human embryonic stem cell reporter lines expressing GFP specifically in neural progenitors. Noisa P, Urrutikoetxea-Uriguen A, Li M, Cui W. Stem Cell Rev. 2010 Sep;6(3):438-49. 10.1007/s12015-010-9159-9 PubMed 20506046
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