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Depositing Lab
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Depositing OrganizationUniversity of Basel
Located in Switzerland -
Citations
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 39210 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 39210-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 39210-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCI-HA
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 4000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemAVO3
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Alt nameMammalian AVO3
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SpeciesM. musculus (mouse)
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MutationG5S and I15V
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Entrez GeneRictor (a.k.a. 4921505C17Rik, 6030405M08Rik, AVO3, D530039E11Rik)
- Promoter CMV
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Tag
/ Fusion Protein
- HA (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site MluI/BssHII (destroyed during cloning)
- 3′ cloning site SmaI (not destroyed)
- 5′ sequencing primer CMV-F
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pCI-HA-based construct (with MluI/BssHII-SmaI sites) that expresses M. musculus mAVO3 N-terminally fused to an HA-epitope.
Addgene's sequencing results found G5S and I15V mutations in the AVO3 sequence when compared to GenBank ID NP_084444.3. This plasmid functions as described in the associated publication.
DNA (Catalog # 39210-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 39210-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCI-HA-mAVO3 (pRL62-3) was a gift from Michael Hall (Addgene plasmid # 39210 ; http://n2t.net/addgene:39210 ; RRID:Addgene_39210) -
For your References section:
Mammalian TOR complex 2 controls the actin cytoskeleton and is rapamycin insensitive. Jacinto E, Loewith R, Schmidt A, Lin S, Ruegg MA, Hall A, Hall MN. Nat Cell Biol. 2004 Nov;6(11):1122-8. Epub 2004 Oct 3. 10.1038/ncb1183 PubMed 15467718