pHAC111-LST8 (pSW75)
(Plasmid
#39211)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 39211 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneYCplac111
- Backbone size w/o insert (bp) 6100
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLST8
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)1700
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Entrez GeneLST8 (a.k.a. YNL006W)
- Promoter LST8
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (unknown if destroyed)
- 3′ cloning site PstI (unknown if destroyed)
- 5′ sequencing primer M13pUC-fwd
- 3′ sequencing primer M13pUC-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Expresses LST8 from LST8 promoter. Cloned as 1.7-kb SacI-PstI fragment into YCplac111. CYC1 terminator (CEN, LEU2)
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHAC111-LST8 (pSW75) was a gift from Michael Hall (Addgene plasmid # 39211 ; http://n2t.net/addgene:39211 ; RRID:Addgene_39211) -
For your References section:
Molecular organization of target of rapamycin complex 2. Wullschleger S, Loewith R, Oppliger W, Hall MN. J Biol Chem. 2005 Sep 2;280(35):30697-704. Epub 2005 Jul 7. 10.1074/jbc.M505553200 PubMed 16002396