Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


pHAC111-LST8 (pSW75)
(Plasmid #39211)

Full plasmid sequence is not available for this item.



Item Catalog # Description Quantity Price (USD)
Plasmid 39211 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 6100
  • Vector type
    Yeast Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
  • Entrez Gene
    LST8 (a.k.a. YNL006W)
  • Promoter LST8

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (unknown if destroyed)
  • 3′ cloning site PstI (unknown if destroyed)
  • 5′ sequencing primer M13pUC-fwd
  • 3′ sequencing primer M13pUC-rev
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Expresses LST8 from LST8 promoter. Cloned as 1.7-kb SacI-PstI fragment into YCplac111. CYC1 terminator (CEN, LEU2)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHAC111-LST8 (pSW75) was a gift from Michael Hall (Addgene plasmid # 39211 ; ; RRID:Addgene_39211)
  • For your References section:

    Molecular organization of target of rapamycin complex 2. Wullschleger S, Loewith R, Oppliger W, Hall MN. J Biol Chem. 2005 Sep 2;280(35):30697-704. Epub 2005 Jul 7. 10.1074/jbc.M505553200 PubMed 16002396