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pFA6a-GST-kanMX6
(Plasmid #39293)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 39293 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pFA6a
  • Backbone size w/o insert (bp) 2300
  • Vector type
    Yeast genomic targeting
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    KanR
  • Alt name
    kanamycin resistance cassette
  • Insert Size (bp)
    2500
  • Tags / Fusion Proteins
    • GST (N terminal on insert)
    • yeast ADH1 terminator (N terminal on insert)
    • A. gossypii translation elongation factor 1a gene promoter (N terminal on insert)
    • A. gossypii translation elongation factor 1a gene terminator (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (not destroyed)
  • 3′ cloning site PmeI (not destroyed)
  • 5′ sequencing primer Sp6_primer
  • 3′ sequencing primer T7_promoter
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFA6a-GST-kanMX6 was a gift from Jurg Bahler & John Pringle (Addgene plasmid # 39293 ; http://n2t.net/addgene:39293 ; RRID:Addgene_39293)
  • For your References section:

    Heterologous modules for efficient and versatile PCR-based gene targeting in Schizosaccharomyces pombe. Bahler J, Wu JQ, Longtine MS, Shah NG, McKenzie A 3rd, Steever AB, Wach A, Philippsen P, Pringle JR. Yeast. 1998 Jul;14(10):943-51. 10.1002/(SICI)1097-0061(199807)14:10<943::AID-YEA292>3.0.CO;2-Y PubMed 9717240