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EKAR2G_design1_mTFP_175_Venus_157
(Plasmid #39829)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 39829 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTriEx4
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 4900
  • Total vector size (bp) 7050
  • Vector type
    Mammalian Expression, Bacterial Expression, Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Erk activity reporter
  • Species
    Synthetic
  • Insert Size (bp)
    2189
  • Promoter CMV
  • Tag / Fusion Protein
    • His-Myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer ctggttattgtgctgtctc
  • 3′ sequencing primer tcagtggtatttgtga
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

A genetically encoded fluorescent sensor of ERK activity. 2008. PNAS 205(49)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EKAR2G_design1_mTFP_175_Venus_157 was a gift from Olivier Pertz (Addgene plasmid # 39829)
  • For your References section:

    A Versatile Toolkit to Produce Sensitive FRET Biosensors to Visualize Signaling in Time and Space. Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O. Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135. 10.1126/scisignal.2004135 PubMed 23882122